mouse neuronal cell line  (ATCC)

Journal: Bioactive Materials

Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury

doi: 10.1016/j.bioactmat.2026.01.022

Figure Lengend Snippet: PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated NSC34 neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.

Article Snippet: NSC34 neuronal culture and transfections: NSC34 neurons (Cedarlane Cat No. CLU140), a hybrid cell line produced by the fusion of motor neuron enriched embryonic mouse spinal cord cells with mouse neuroblastoma cells, were cultured in growth medium Dulbecco's Modified Eagle Medium (DMEM; Sigma-Aldrich, Ireland) supplemented with 10 % fetal bovine serum (FBS; Labtech UK), 1 % (v/v) L-Glutamine (Sigma-Aldrich, Ireland), and 1 % (v/v) Penicillin- Streptomycin Solution (Sigma-Aldrich, Ireland) in a T-175 cell culture flask (37 °C, 5 % CO 2 ).

Techniques: Transfection, Knockdown, One-tailed Test, Expressing, Activity Assay, Control, Staining

Journal: Bioactive Materials

Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury

doi: 10.1016/j.bioactmat.2026.01.022

Figure Lengend Snippet: Scaffold-mediated siRNA delivery does not affect the viability of neurons, results in prolonged downregulation of PTEN, and stimulates increased expression of BCL2 and GAP43 mRNA . (A) The metabolic activity of NSC34 neurons cultured on PTEN -siRNA activated scaffolds peaked by day 3 post-transfection [t(2) = 4.0599, p = 0.05565, 95 % CI 93.954 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.

Article Snippet: NSC34 neuronal culture and transfections: NSC34 neurons (Cedarlane Cat No. CLU140), a hybrid cell line produced by the fusion of motor neuron enriched embryonic mouse spinal cord cells with mouse neuroblastoma cells, were cultured in growth medium Dulbecco's Modified Eagle Medium (DMEM; Sigma-Aldrich, Ireland) supplemented with 10 % fetal bovine serum (FBS; Labtech UK), 1 % (v/v) L-Glutamine (Sigma-Aldrich, Ireland), and 1 % (v/v) Penicillin- Streptomycin Solution (Sigma-Aldrich, Ireland) in a T-175 cell culture flask (37 °C, 5 % CO 2 ).

Techniques: Expressing, Activity Assay, Cell Culture, Transfection, Control, Lactate Dehydrogenase Assay, Gene Expression, One-tailed Test

Journal: ACS Omega

Article Title: Research on the Molecular Mechanism of Ginsenoside Ro in Neuronal Damage Following Subarachnoid Hemorrhage

doi: 10.1021/acsomega.5c13079

Figure Lengend Snippet: Effect of Ginsenoside Ro on the mRNA expressions of Bcl-2, EGFR, MMP9, and SRC in HT22 cells (x̅ ± s, n = 3). The mRNA expression levels of Bcl-2, EGFR, MMP9, and SRC between controls and Ro treatment groups were detected by real-time quantitative polymerase chain reaction (A–D). Effect of Ginsenoside Ro on the protein expressions of Bcl-2, EGFR (E–G). Results are shown as x̅ ± s, n = 3, * p < 0.05.

Article Snippet: The mouse hippocampal neuron cell line HT22 ( Mus musculus hippocampal neuron cell line HT22) was purchased from Wuhan Procell Life Technology Co., Ltd. Ginsenoside Ro (CAS No. B21068 , purity ≥98%) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: ACS Omega

Article Title: Research on the Molecular Mechanism of Ginsenoside Ro in Neuronal Damage Following Subarachnoid Hemorrhage

doi: 10.1021/acsomega.5c13079

Figure Lengend Snippet: Protective effect of Ginsenoside Ro on hemoglobin-induced HT22 cell damage (x̅ ± s, n = 3). Cell viability was detected by CCK-8 (A); The protein expression of Bcl-2 was detected by Western blot (B). SAH vs Control group,* p < 0.05; SAH vs Ro (100 μM)+SAH, # p < 0.05.

Article Snippet: The mouse hippocampal neuron cell line HT22 ( Mus musculus hippocampal neuron cell line HT22) was purchased from Wuhan Procell Life Technology Co., Ltd. Ginsenoside Ro (CAS No. B21068 , purity ≥98%) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: CCK-8 Assay, Expressing, Western Blot, Control